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polybrene  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology polybrene
    Polybrene, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 2407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polybrene/Polybrene/pm42881414-62-0-1
    Average 96 stars, based on 2407 article reviews
    polybrene - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transduction:

    Article Title: A genome-wide CRISPR knockout screen identifies host dependency factors and potential viral-induced cell death regulators for porcine deltacoronavirus infection
    Article Snippet: The supernatants collected at 24 and 48 hours post transfection were pooled, filtered through sterile 0.22 μm PES syringe filters (Millipore), aliquoted and stored at -80°C. .. Transduction was performed in the presence of 8 μg/mL polybrene (Santa Cruz Biotechnology). ..

    Article Title: A Toxoplasma gondii-derived ROP38 protein engages Ser15/Thr18-linked p53 activation and DNA damage-associated apoptotic signaling in human cancer cells.
    Article Snippet: Restriction enzymes and ligases utilized included PspXI (NEB, Ipswich, MA, USA; R0656S), BamHI (Enzynomics, Daejeon, Republic of Korea; R003S), and T4 DNA ligase (Enzynomics; M001S). .. Polybrene (Santa Cruz Biotechnology, Dallas, TX, USA; sc-134220) was used for lentiviral transduction. .. Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology; sc-2003) were used for recombinant protein-based interaction assays.

    Article Title: Gα12/13 deficiency attenuates obesity-associated adipose tissue inflammation and promotes energy expenditure
    Article Snippet: Lentiviral particles were produced by co-transfecting HEK293T cells with pMD2.G (Addgene, #12259), psPAX2 (Addgene, #12260), and the respective lentiviral transfer plasmid constructs in a 1:3:4 ratio using the JetPrime Kit (Polyplus, 114-15). .. After 48 h, supernatants were collected, filtered through 0.45-μm filter, and supplemented with 8μg/mL polybrene (Santa Cruz Biotechnology, 134220) for transduction. .. Transduced cells were selected using puromycin (Santa Cruz Biotechnology, sc-108071A) and blasticidin (SigmaAldrich, SBR00022).

    Article Title: From Platform Development to Scale-Up: Stable Producer Cell Line-Based Lentiviral Vector Manufacturing
    Article Snippet: .. Functional LVV titers were determined by transduction of HEK293T cells with serial dilutions of viral supernatants in the presence of 8 μg/mL of Polybrene (Santa Cruz Biotechnology Inc., Dallas, TX, USA). .. Transduced cells were cultured for 72 h, after which transduction units were measured by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA, USA) based on GFP expression, and viral titers were calculated accordingly.

    Virus:

    Article Title: Targeting mitochondrial EGFR by CTAB in triple-negative breast cancer
    Article Snippet: .. Collect virus supernatant at 48 and 72 h after transfection, filter and concentrate through Amicon Ultra ultrafiltration centrifuge tube (Millipore, UFC910024), add 1 μg/mL Polybrene (Santa Cruz, sc-134220) and infect target cells. ..

    Transfection:

    Article Title: Targeting mitochondrial EGFR by CTAB in triple-negative breast cancer
    Article Snippet: .. Collect virus supernatant at 48 and 72 h after transfection, filter and concentrate through Amicon Ultra ultrafiltration centrifuge tube (Millipore, UFC910024), add 1 μg/mL Polybrene (Santa Cruz, sc-134220) and infect target cells. ..

    other:

    Article Title: PHYHD1-mediated translational control regulates renal cell carcinoma progression.
    Article Snippet: Article PHYHD1-mediated translational control regulates renal cell carcinoma progression

    Stable Transfection:

    Article Title: TMBIM family proteins regulate neuronal differentiation through modulation of ER and lysosomal Ca²⁺ homeostasis
    Article Snippet: .. To establish stable cell lines of P19 knockdown TMBIM families, cells were incubated with 8 μg/mL polybrene (Santa Cruz Biotechnology, Dallas, TX, USA) and lentiviral particles harbouring each gene, followed by selection with puromycin dihydrochloride (Santa Cruz Biotechnology, Dallas, TX, USA) for 1 week. ..

    Knockdown:

    Article Title: TMBIM family proteins regulate neuronal differentiation through modulation of ER and lysosomal Ca²⁺ homeostasis
    Article Snippet: .. To establish stable cell lines of P19 knockdown TMBIM families, cells were incubated with 8 μg/mL polybrene (Santa Cruz Biotechnology, Dallas, TX, USA) and lentiviral particles harbouring each gene, followed by selection with puromycin dihydrochloride (Santa Cruz Biotechnology, Dallas, TX, USA) for 1 week. ..

    Incubation:

    Article Title: TMBIM family proteins regulate neuronal differentiation through modulation of ER and lysosomal Ca²⁺ homeostasis
    Article Snippet: .. To establish stable cell lines of P19 knockdown TMBIM families, cells were incubated with 8 μg/mL polybrene (Santa Cruz Biotechnology, Dallas, TX, USA) and lentiviral particles harbouring each gene, followed by selection with puromycin dihydrochloride (Santa Cruz Biotechnology, Dallas, TX, USA) for 1 week. ..

    Selection:

    Article Title: TMBIM family proteins regulate neuronal differentiation through modulation of ER and lysosomal Ca²⁺ homeostasis
    Article Snippet: .. To establish stable cell lines of P19 knockdown TMBIM families, cells were incubated with 8 μg/mL polybrene (Santa Cruz Biotechnology, Dallas, TX, USA) and lentiviral particles harbouring each gene, followed by selection with puromycin dihydrochloride (Santa Cruz Biotechnology, Dallas, TX, USA) for 1 week. ..

    Functional Assay:

    Article Title: From Platform Development to Scale-Up: Stable Producer Cell Line-Based Lentiviral Vector Manufacturing
    Article Snippet: .. Functional LVV titers were determined by transduction of HEK293T cells with serial dilutions of viral supernatants in the presence of 8 μg/mL of Polybrene (Santa Cruz Biotechnology Inc., Dallas, TX, USA). .. Transduced cells were cultured for 72 h, after which transduction units were measured by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA, USA) based on GFP expression, and viral titers were calculated accordingly.

    Negative Control:

    Article Title: CHCHD2 mitigates age-associated proteostatic collapse by maintaining homeostasis of the mitochondria-lysosome axis.
    Article Snippet: .. HT22 mouse hippocampal cells were transduced with MTSBirA, MTS-BirA-CHCHD2WT, or MTS-BirA-CHCHD2T61I lentivirus with 10 μg/mL polybrene (Santa Cruz Biotechnology, sc-134220) for 48 h. Stable cells were selected with 10 μg/mL of puromycin (ThermoFisher, A1113803) until the negative control plate of non-transduced HT22 cells fully died. ..



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